{"id":173,"date":"2025-05-27T19:00:13","date_gmt":"2025-05-27T19:00:13","guid":{"rendered":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/kwon-research-group\/?p=173"},"modified":"2025-05-27T19:05:00","modified_gmt":"2025-05-27T19:05:00","slug":"production-of-paclitaxel-loaded-peg-b-pla-micelles-using-peg-for-drug-loading-and-freeze-drying","status":"publish","type":"post","link":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/kwon-research-group\/2025\/05\/27\/production-of-paclitaxel-loaded-peg-b-pla-micelles-using-peg-for-drug-loading-and-freeze-drying\/","title":{"rendered":"Production of paclitaxel-loaded PEG-b-PLA micelles using PEG for drug loading and freeze-drying"},"content":{"rendered":"
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October 1, 2022<\/div>\r\nCite this<\/a>\r\n\r\n<\/div>\r\n
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Abstract<\/h2>\r\n
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https:\/\/doi.org\/10.1016\/j.jconrel.2022.08.032<\/figcaption><\/figure>\r\n\r\nA new approach named PEG-assist is introduced for the production of drug-loaded polymeric\u00a0micelles<\/a>. The method is based on the use of\u00a0PEG<\/a>\u00a0as the non-selective solvent for PEG-b<\/em>-PLA in the fabrication procedure. Both hydration temperature and PEG molecular weight are shown to have a significant effect on the encapsulation efficiency of\u00a0PTX<\/a>\u00a0in PEG4kDa<\/sub>\u2013b<\/em>-PLA2kDa<\/sub>\u00a0micelles. The optimal procedure for fabrication includes the use of PEG1kDa<\/sub>\u00a0as the solvent at 60\u00a0\u00b0C, cooling the mixture to 40\u00a0\u00b0C, hydration at 40\u00a0\u00b0C, freezing at \u221280\u00a0\u00b0C and freeze-drying at \u221235\u00a0\u00b0C, 15\u00a0Pa. No significant difference (p<\/em>\u00a0>\u00a00.05) in PTX encapsulation, average particle size and polydispersity index is observed between the samples before freeze-drying and after reconstitution of the freeze-dried cake. The prepared PTX formulations are stable at room temperature for at least 8\u00a0h. Scaling the batch size to 25\u00d7 leads to no significant change (p<\/em>\u00a0>\u00a00.05) in PTX encapsulation, average particle size and polydispersity index. PEG-assist method is applicable to other drugs such as 17-AAG, and\u00a0copolymers<\/a>\u00a0of varied molecular weights. The use of no\u00a0organic solvent<\/a>, simplicity, cost-effectiveness, and efficiency makes PEG-assist a very promising approach for large scale production of drug-loaded polymeric micelles.\r\n

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