{"id":317,"date":"2021-07-28T19:35:05","date_gmt":"2021-07-28T19:35:05","guid":{"rendered":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/?p=317"},"modified":"2025-08-18T17:39:52","modified_gmt":"2025-08-18T17:39:52","slug":"size-dependent-drug-loading-gene-complexation-cell-uptake-and-transfection-of-a-novel-dendron-lipid-nanoparticle-for-drug-gene-co-delivery","status":"publish","type":"post","link":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/2021\/07\/28\/size-dependent-drug-loading-gene-complexation-cell-uptake-and-transfection-of-a-novel-dendron-lipid-nanoparticle-for-drug-gene-co-delivery\/","title":{"rendered":"Size-Dependent Drug Loading, Gene Complexation, Cell Uptake, and Transfection of a Novel Dendron-Lipid Nanoparticle for Drug\/Gene Co-delivery"},"content":{"rendered":"

Abstract<\/h2>\r\n
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Dendron micelles have shown promising results as a multifunctional delivery system, owing to their unique molecular architecture. Herein, we have prepared a novel poly(amidoamine) (PAMAM) dendron-lipid hybrid nanoparticle (DLNP) as a nanocarrier for drug\/gene co-delivery and examined how the dendron generation of DLNPs impacts their cargo-carrying capabilities. DLNPs, formed by a thin-layer hydration method, were internally loaded with chemo-drugs and externally complexed with plasmids. Compared to generation 2 dendron DLNP (D2LNPs), D3LNPs demonstrated a higher drug encapsulation efficiency (31% vs 87%) and better gene complexation (minimal N\/P ratio of 20:1 vs 5:1 for complexation) due to their smaller micellar aggregation number and higher charge density, respectively. Furthermore, D3LNPs were able to avoid endocytosis and subsequent lysosomal degradation and demonstrated a higher cellular uptake than D2LNPs. As a result, D3LNPs exhibited significantly enhanced antitumor and gene transfection efficacy in comparison to D2LNPs. These findings provide design cues for engineering multifunctional dendron-based nanotherapeutic systems for effective combination cancer treatment.<\/p>\r\n\"visualization\r\n\r\n

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Cited by<\/h2>\r\nThis article is cited by 2 publications\r\n
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  1. \r\n
    Poellmann, M. J., Rawding, P., Kim, D., Bu, J., Kim, Y., & Hong, S. (2022). Branched, dendritic, and hyperbranched polymers in liquid biopsy device design.\u00a0Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology<\/i>,\u00a014<\/i>(3), e1770.\u00a0https:\/\/doi.org\/10.1002\/wnan.1770<\/a><\/div><\/li>\r\n \t
  2. \r\n
    Nair, A., Bu, J., Rawding, P. A., Do, S. C., Li, H., & Hong, S. (2021). Cytochalasin B Treatment and Osmotic Pressure Enhance the Production of Extracellular Vesicles (EVs) with Improved Drug Loading Capacity.\u00a0Nanomaterials (Basel, Switzerland)<\/i>,\u00a012<\/i>(1), 3.\u00a0https:\/\/doi.org\/10.3390\/nano12010003<\/a><\/div><\/li>\r\n<\/ol>\r\n<\/figure>\r\n<\/div>","protected":false},"excerpt":{"rendered":"Abstract Dendron micelles have shown promising results as a multifunctional delivery system, owing to their unique molecular architecture. Herein, we have prepared a novel poly(amidoamine) (PAMAM) dendron-lipid hybrid nanoparticle (DLNP) as a nanocarrier for drug\/gene …","protected":false},"author":7,"featured_media":682,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-317","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications"],"acf":[],"_links":{"self":[{"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/317","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/comments?post=317"}],"version-history":[{"count":1,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/317\/revisions"}],"predecessor-version":[{"id":320,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/posts\/317\/revisions\/320"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/media\/682"}],"wp:attachment":[{"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/media?parent=317"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/categories?post=317"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/wwwtest.pharmacy.wisc.edu\/faculty\/hong-research-group\/wp-json\/wp\/v2\/tags?post=317"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}